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Johan Lindgren

Johan Lindgren

Universitetslektor

Johan Lindgren

Microspectroscopic evidence of cretaceous bone proteins.

Författare

  • Johan Lindgren
  • Per Uvdal
  • Anders Engdahl
  • Andrew H Lee
  • Carl Alwmark
  • Karl-Erik Bergquist
  • Einar Nilsson
  • Peter Ekström
  • Magnus Rasmussen
  • Desiree Douglas
  • Michael J Polcyn
  • Louis L Jacobs

Summary, in English

Low concentrations of the structural protein collagen have recently been reported in dinosaur fossils based primarily on mass spectrometric analyses of whole bone extracts. However, direct spectroscopic characterization of isolated fibrous bone tissues, a crucial test of hypotheses of biomolecular preservation over deep time, has not been performed. Here, we demonstrate that endogenous proteinaceous molecules are retained in a humerus from a Late Cretaceous mosasaur (an extinct giant marine lizard). In situ immunofluorescence of demineralized bone extracts shows reactivity to antibodies raised against type I collagen, and amino acid analyses of soluble proteins extracted from the bone exhibit a composition indicative of structural proteins or their breakdown products. These data are corroborated by synchrotron radiation-based infrared microspectroscopic studies demonstrating that amino acid containing matter is located in bone matrix fibrils that express imprints of the characteristic 67 nm D-periodicity typical of collagen. Moreover, the fibrils differ significantly in spectral signature from those of potential modern bacterial contaminants, such as biofilms and collagen-like proteins. Thus, the preservation of primary soft tissues and biomolecules is not limited to large-sized bones buried in fluvial sandstone environments, but also occurs in relatively small-sized skeletal elements deposited in marine sediments.

Avdelning/ar

  • Berggrundsgeologi
  • Kemisk fysik
  • MAX IV-laboratoriet
  • Centrum för analys och syntes
  • Funktionell zoologi
  • Infektionsmedicin
  • Cellpatologi, Malmö

Publiceringsår

2011

Språk

Engelska

Publikation/Tidskrift/Serie

PLoS ONE

Volym

6

Issue

4

Dokumenttyp

Artikel i tidskrift

Förlag

Public Library of Science

Ämne

  • Infectious Medicine

Status

Published

Forskningsgrupp

  • Cell Pathology, Malmö

ISBN/ISSN/Övrigt

  • ISSN: 1932-6203